Last year Scunthorpe reached the northern quarter-finals, where they lost to Hereford United.
Unlike recent seasons, top clubs from the Conference are no longer involved.
And extra-time has been scrapped, with ties that are level after 90 minutes going straight to a penalty shootout.
Draw in full
Northern Section (West)
Accrington Stanley v Carlisle
Wrexham v Rochdale
Macclesfield v Stockport
Bury v Tranmere
Northern Section (East)
Hartlepool v Rotherham
Huddersfield v Doncaster
Bradford v Scunthorpe
Lincoln v Grimsby
Southern Section (East)
Brighton v Boston
Northampton v Brentford
Notts County v Barnet
Gillingham v Notts Forest
Southern Section (West)
Wycombe v Swindon
Walsall v Swansea
Hereford v Shrewsbury
Bristol Rovers v Torquay