Aston Villa, who knocked Scunthorpe out of the competition on Wednesday, will travel to Leicester.
It marks a quick return to the Foxes for Villa boss Martin O'Neill, who twice won the competition while manager at Filbert Street.
Chesterfield's reward for dumping Manchester City out of the competiton is another home game against top-flight opposition - this time West Ham will travel to Saltergate.
Doncaster, who beat Championship side Derby County in midweek, will travel to League Two side Wycombe Wanderers.
Draw in full:
Wycombe v Doncaster
Sheffield United v Birmingham
Leicester v Aston Villa
Watford v Hull
Leeds v Southend
Chesterfield v West Ham
Notts County v Southampton
Newcastle v Portsmouth
Charlton v Bolton
Liverpool v Reading
West Brom v Arsenal
Port Vale v Norwich
Everton v Luton
MK Dons v Tottenham
Crewe v Manchester United
Blackburn v Chelsea
Port Vale will host Norwich City.