Clubs in the second tier of English football must provide "future financial information" ahead of each season and show they have no overdue transfer or compensation fees, employee wages or income tax and national insurance contributions.
The Football League said clubs that are in default or "business plans that cast doubt on their ability to fulfil fixtures or pay football creditors" will be subject to "budget constraints" including a possible embargo on signing players.
"I am pleased that our clubs have supported new and revised regulations that further strengthen the professionalism and good governance of The Football League," said Greg Clarke, chairman of the Football League.
"We will be doing everything possible to support our clubs through a difficult economic environment and to encourage more and more fans to enjoy the fantastic product offered on the pitch by all 72 Football League clubs."
These were one of several new rules on reporting finances adopted by clubs at an extraordinary general meeting held at Derby County's Pride Park stadium.
They have created an annual reporting process for all club directors, who now cannot take up any role at a club without clearance from the Football League.
People prevented from the United Kingdom or having funds here cannot own an interest in a club.
Clubs must now publish the identity of any individual owning 10 per cent of a club or more.